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Effects of tianeptine sodium(CAS 30123-17-2) on hippocampal neurons in rat model of depression

Date : 2016/8/12 11:02:48

Study the effects of fluoxetine (FLU) and tianeptine (TIA) of different levels of neurotransmitters in rat hippocampal tianeptine sodium(CAS 30123-17-2) synaptophysine. Depression Model (SYN), neuron-specific enolase (NSE ) and glial fibrillary acidic protein (GFAP) expression in action and observe before stress after stress and long-term by antidepressants (4w), after treatment of rat hippocampal ultrastructure and behavioral changes, and to explore for antidepressants the role of the hippocampus in rats model of depression, and depression can pathophysiological mechanisms. Methods: 50 rats were randomly divided into two groups 10, 40 in the experimental group (wherein the stress group, saline control group, fluoxetine group and tianeptine group each 10), all experimental groups 40 large use mouse singly housed with chronic unpredictable mild stress (CUMS) jointly established model of depression.
Investigate the effect of fluoxetine and tianeptine sodium(CAS 30123-17-2) hippocampus of chronic stress rats of protein kinase C (PKC) expression. Methods 60 male Wistar rats were randomly divided into model of depression, fluoxetine, thiophene Kneipp statin group and normal control group, 15 rats in each model group, fluoxetine group and tianeptine rats given stressors for 21 d, the control group during normal feeding, fluoxetine daily irrigation during stimulation stomach fluoxetine (10 mg / kg), tianeptine group were fed daily tianeptine thiophene (50 mg / kg), 0.9% in the model group and normal control group were fed a daily volume of sodium chloride injection, etc. fluid. the opening behavioral test application method and the liquid consuming experiments using Western- expression of PKC in hippocampus of rats blotting analysis. results of stress, the level of model group across the grid, rearing, modification times, sugar the percentage of consumption was significantly lower than the control group (P <0.01); modification times and sucrose consumption percentage was significantly higher than the model group (P <0.05); tianeptine group sucrose consumption percentage is significantly higher than the model group (P <0.05) in the Western-blotting assay, the expression levels of PKC in hippocampus of rats in model group was significantly lower than the control group (P <0.01);. fluoxetine hippocampal PKC expression was significantly higher than the model group (P <0.01), and the normal control group, the difference was not statistically significant (P> 0.05); PKC in hippocampus of rats tianeptine expression was significantly higher than the model group (P <0.01), but still significantly lower than the control group (P <0.01) Conclusion: fluoxetine can be reversed to reduce chronic stress depression model rat hippocampus PKC expression; tianeptine sodium(CAS 30123-17-2) can partially reverse chronic stress model of depression PKC expression in rat hippocampus reduced.